Composite

Part:BBa_K5143028

Designed by: Perrel Aymeric   Group: iGEM24_UnivLyon1-INSALyon   (2024-09-27)
Revision as of 15:55, 27 September 2024 by Registry (Talk | contribs)

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-- No description --

The final part pUC57-5'URA-GAP_promoter-alphafactorV1-plu1537-dCBD-TDH1_term-3'URA is composed of the plu1537 gene is a antitermite toxin (known as Bt toxin) fused to a dCBD and an alphafactor signal.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1927
    Illegal EcoRI site found at 5366
    Illegal EcoRI site found at 5893
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5366
    Illegal EcoRI site found at 5893
    Illegal SpeI site found at 1928
    Illegal PstI site found at 1942
    Illegal NotI site found at 1935
    Illegal NotI site found at 5899
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5366
    Illegal EcoRI site found at 5893
    Illegal XhoI site found at 3531
    Illegal XhoI site found at 5372
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1928
    Illegal EcoRI site found at 5366
    Illegal EcoRI site found at 5893
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5893
    Illegal EcoRI site found at 5366
    Illegal SpeI site found at 1928
    Illegal PstI site found at 1942
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2640


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