Composite

Part:BBa_K5398631

Designed by: ziyi guo   Group: iGEM24_NAU-CHINA   (2024-09-27)
Revision as of 15:27, 27 September 2024 by Registry (Talk | contribs)

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Use RAT terminator sequence to improve the leakage BBa_K3739064 system

This part is to lower the leakage level of the BBa_K3739064. Aiming to control the leakage issue, we insert the RAT terminator sequence into the regions between the pBlind promoter sequence and superfolder(sfGFP) reporter gene sequence. Besides, the expression level of LicV is high enough to ensure that the sfGFP can transcript normally due to the strong promoter J23104.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 379
    Illegal BamHI site found at 197
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 255
    Illegal AgeI site found at 365
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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