Coding

Part:BBa_K5335000

Designed by: Zekun Luo   Group: iGEM24_HZAU-China   (2024-09-27)
Revision as of 14:06, 27 September 2024 by Registry (Talk | contribs)

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MS2 CP tandem dimer fusion containing SpyTag tag

The sequence consists of two MS2 capsid protein subunits in tandem and a Spytag peptide segment inserted between the two subunits. The protein can correctly self-assemble to form VLP particles of about 25~27nm, and the Spytag on its surface can combine with Spycatcher to display some functional proteins in the periphery of the particles and become a delivery platform.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 331
    Illegal BsaI site found at 760


[edit]
Categories
Parameters
None