Coding
Part:BBa_K5335000
Designed by: Zekun Luo Group: iGEM24_HZAU-China (2024-09-27)
MS2 CP tandem dimer fusion containing SpyTag tag
The sequence consists of two MS2 capsid protein subunits in tandem and a Spytag peptide segment inserted between the two subunits. The protein can correctly self-assemble to form VLP particles of about 25~27nm, and the Spytag on its surface can combine with Spycatcher to display some functional proteins in the periphery of the particles and become a delivery platform.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 331
Illegal BsaI site found at 760
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Categories
Parameters
None |