Part:BBa_K5143020

AGA2, a pre-peptide that is secreted by the Saccharomyces cerevisiae yeast

Description

AGA2 is a pre-peptide that is secreted by the Saccharomyces cerevisiae yeast. When fused with another protein, AGA2 enables the secretion of the whole protein in the extracellular medium.

Figure 1: AGA2 coding sequence

Figure 2: The fluorescence of mRuby is assessed in either the supernatant or within the cells to determine whether the protein is produced and secreted by AGA2

Construction

AGA2 was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with the mRuby chromoprotein in order to color the yeast culture in red and so to test the activation of the pADH1 promoter: BBa_K5143021

References

Cappellaro, C. et al. Saccharomyces cerevisiae a- and alpha-agglutinin: characterization of their molecular interaction. The EMBO Journal 10, 4081–4088 (1991) https://doi.org/10.1002/j.1460-2075.1991.tb04984.x

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 256
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 226
  Illegal AgeI site found at 265
  
• 1000
  COMPATIBLE WITH RFC[1000]