Part:BBa_K5184002
ShZIS
Targeting both the prevention and extermination of T. urticae, we aim to produce 7-epi-zingiberene (7epiZ), a sesquiterpene that is found to have repellent, fecundity-reducing, and fatal effects towards T. urticae. In order to continue to synthesize 7epiZ after the production of Z,Z-FPP, we introduce ShZIS, a sesquiterpene synthase which catalyzes the reaction of 2Z,6Z-FPP and converts it into 7epiZ. Through discovering and exploring the catalytic effects of ShZIS, we provide future iGEM teams with a novel and effective biological catalyst able to produce a sesquiterpene from an isoprenyl diphosphate.
Usage and Biology
ShZIS is a sesquiterpene synthase derived from Solanum habrochaites (an accession of the wild tomato species), and is present and expressed in the tomato's glandular trichomes. By utilizing three isoprenoid units, ShZIS catalyzes the formation of sesquiterpenes, a compound with 15 carbons. Intermediates that are commonly used include isopentenyl diphosphate (IPP), dimethylallyl diphosphate (DMAPP), and (E,E)-α-farnesyl diphosphate (FPP). Originally, it is used to produce secondary metabolites that don't direcly interfere plant growth but are useful in fragrance to repell and exterminate pests. We constructed a novel sesquiterpene synthesis pathway in E. coli. Using glucose as our raw material, we introduce the MVA pathway, which transforms glucose into dimethylallyl diphosphate (DMAPP) and isopentenyl diphosphate (IPP). Afterwards, SltNPPS, a neryl diphosphate synthase catalyze the production of NPP from IPP and DMAPP. Mvan4662 is then introduced to catalyze the formation of Z,Z-FPP. In the end, ShZIS transforms Z,Z-FPP into 7epiZ.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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