Coding

Part:BBa_K5034212:Design

Designed by: Zongyu Guo   Group: iGEM24_Nanjing-China   (2024-09-16)
Revision as of 11:34, 27 September 2024 by Ji-yf (Talk | contribs)


PolyP + AMP --> ADP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in Shewanella and regulating cellular phosphorus metabolism and electron transfer.


Source

Phosphate-AMP phosphotransferase(PAP) from Acinetobacter johnsonii. NCBI reference sequence: AB092983.1

References

Itoh, H., & Shiba, T. (2004). Polyphosphate synthetic activity of polyphosphate:AMP phosphotransferase in Acinetobacter johnsonii 210A. Journal of Bacteriology, 186(15), 5178-5181. doi:10.1128/jb.186.15.5178-5181.2004