Device

Part:BBa_K5477043

Designed by: Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-27)
Revision as of 11:00, 27 September 2024 by Registry (Talk | contribs)

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Biosensor device I for detection of BPA

In this system, the pRET2-LexA-ERα(LBD) receptor module responds to the presence of estrogen or estrogen-like compounds, in this case BPA. When these ligands bind to the ERα LBD, it triggers a transcriptional response by allowing the LexA-ERα chimeric activator to bind to the Lex6Op sequences in the pLex6Op-pLEU2-NanoLuc reporter module. This activates the pLEU2 promoter, leading to the expression of NanoLuc, which produces a luminescent signal. The intensity of the luminescence is proportional to the concentration of the estrogenic compound in the environment, providing a measurable output of receptor activation.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1606
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 182
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1740
    Illegal BglII site found at 2751
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1606
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1606
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
    Illegal AgeI site found at 37
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None