Coding

Part:BBa_K5034205:Design

Designed by: Zongyu Guo   Group: iGEM24_Nanjing-China   (2024-08-28)
Revision as of 10:45, 27 September 2024 by Ji-yf (Talk | contribs)


PolyP <->Pi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in Shewanella and regulating cellular phosphorus metabolism and electron transfer.


Source

Polyphosphate kinase 2(PPK2) from Pseudomonas paraeruginosa. NCBI reference sequence: NZ_CP020560.1.

References

ZHANG H, ISHIGE K, KORNBERG A 2002. A polyphosphate kinase (PPK2) widely conserved in bacteria. Proceedings of the National Academy of Sciences [J], 99: 16678-16683.