Composite

Part:BBa_K5241007

Designed by: yingmei tang   Group: iGEM24_ECIB-PKU   (2024-09-25)
Revision as of 07:58, 27 September 2024 by Supergrc (Talk | contribs)


PelB-PkhppD-6xH-20b（+）

Based on Pseudomonas_koreensis_D26 [gene=hppD][protein=4-hydroxyphenylpyruvate dioxygenase], a gene with a PelB signal peptide and 6His tag is synthesized, aiming for the tyrosinase to be localized to the bacterial periplasmic space.

Result:

When attempting to induce the expression of PeLB-PkhppD-6XH for melanin production in Escherichia coli BL21 (DE3) / BL21 (DE3) pLys using L-arabinose, the expression outcome was not as effective as that of the hppD gene , with only a small amount of melanin being expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 967
    Illegal PstI site found at 925
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 967
    Illegal PstI site found at 925
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 967
    Illegal XhoI site found at 1075
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 967
    Illegal PstI site found at 925
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 967
    Illegal PstI site found at 925
    Illegal NgoMIV site found at 342
    Illegal NgoMIV site found at 1140
    Illegal AgeI site found at 319
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 316


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