Coding

Part:BBa_K5436106

Designed by: Shota Yamamoto, Yuto Toriyama, Jun Kawabata, Ryoto Miyamoto, Hanna Watanabe, Ryojun Hayashizaki   Group: iGEM24_Waseda-Tokyo   (2024-09-27)
Revision as of 04:30, 27 September 2024 by Rhayashizaki (Talk | contribs)


Optimized RBS+BIND-PETase(ID6)

BIND-System Module(BBa_K5436100)+PETase(ID6)(BBa_K5436012)

BIND-PETase equipped with In silico Evolution PETase(ID6), which doesn’t work at all as whole-cell biocatalysts.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 395
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 395
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 478
    Illegal XhoI site found at 912
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 395
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 395
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None