Coding

Part:BBa_K5321018

Designed by: Yiyan Liao   Group: iGEM24_Peking   (2024-09-27)
Revision as of 02:43, 27 September 2024 by Registry (Talk | contribs)

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βhCG_PPV cleavage site_GST

βhCG_PPV cleavage site_GST is the substrate of PPVp in output module, and it is immobilized on a GST affinity chromatography column. Then, when our sample flows through the column, if the split-PPVp is active in the sample, cleavage happens. hCG flows out into the hCG colloidal test paper. The test paper will show a positive result. If there isn't any active PPVp in the sample, then the hCG colloidal test paper will show a negative result.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 691
    Illegal NgoMIV site found at 874
    Illegal NgoMIV site found at 918
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1008
    Illegal SapI.rc site found at 85


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Categories
Parameters
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