Device

Part:BBa_K5152008

Designed by: Tian Xin Ho   Group: iGEM24_HongKong-JSS   (2024-09-24)
Revision as of 00:10, 27 September 2024 by Pok430 (Talk | contribs)

MerR-pMerT mercury sensing chromoprotein reporter device

This device contains the mercury-inducible promoter pMerT and co-expresses its repressor protein MerR, which specifically binds to mercury. When mercury is present, the pMerT promoter drives the expression of the chromoprotein tsPurple, serving as the reporter signal. The design was inspired by iGEM 2010 Team Peking (BBa_K346002).

We used tsPurple as the reporter gene, a purple chromoprotein chosen for its visible colour. This helps create a user-friendly and cost-effective mercury biosensor without needing expensive equipment or special techniques.

However, because we ordered the wrong DNA, we didn't have time to clone this construct, and no experiments were conducted on it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 35
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None