Device

Part:BBa_K5152005

Designed by: Tsz Yan Lai   Group: iGEM24_HongKong-JSS   (2024-09-24)
Revision as of 00:02, 27 September 2024 by Pok430 (Talk | contribs)

pCadA cadmium sensing chromoprotein reporter device

This part was used to create a cadmium-sensing biosensor for our project. The design of this part was inspired by iGEM 2009 Team Newcastle (BBa_K174015).

Our goal is to develop heavy metal biosensors that don't require special techniques or expensive equipment, so we used chromoproteins for their visible colours.

We designed this part and ordered it from IDT gBlock. However, we consistently failed to obtain successful transformants for unknown reasons. Despite using the same assembly process as other constructs, which succeeded, this one did not. The cause remains unclear.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 228
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None