Translational_Unit
Part:BBa_K5490017
Designed by: IOANNIS VASILEIOS ELAFROPOULOS Group: iGEM24_IOANNINA (2024-09-24)
Revision as of 22:46, 26 September 2024 by Tzonissss13 (Talk | contribs)
Ca-Dependent Synthetic NF-AT
Is a synthetic NFAT transcription factor, after an increase in calcium, will enter the nucleus and bind to a specific minimal promoter. It uses the TALE system for binding to DNA and VP16 as the activation domain. Under homeostatic conditions, it is anchored in the plasma membrane via the KRφ peptide. It has both Myc and FLAG tags for immunohistochemical analysis and Western blotting
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 723
Illegal EcoRI site found at 766
Illegal EcoRI site found at 1926
Illegal SpeI site found at 741
Illegal PstI site found at 1744
Illegal PstI site found at 1998 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 723
Illegal EcoRI site found at 766
Illegal EcoRI site found at 1926
Illegal SpeI site found at 741
Illegal PstI site found at 1744
Illegal PstI site found at 1998
Illegal NgoMIV site found at 1198
Illegal NgoMIV site found at 1218
Illegal NgoMIV site found at 1234
Illegal NgoMIV site found at 1699
Illegal NgoMIV site found at 1983 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |