Composite
Part:BBa_K5477028
Designed by: Kate Malana Escobar Group: iGEM24_UCopenhagen (2024-09-26)
pPOP6-LexA-ERα(LBD) - receptor module
The pPOP6-LexA-ERα(LBD) composite part is a fusion module that combines the pPOP6 promoter with the LexA-ERα(LBD) construct. In this system, the pPOP6 promoter drives the expression of the LexA-ERα(LBD) fusion protein, which consists of the LexA DNA-binding domain (DBD) fused to the ligand-binding domain (LBD) of Estrogen Receptor Alpha (ERα). This design allows for precise ligand-dependent regulation of our reporter module based on the presence of estrogen or estrogen-like compounds like BPA. Upon binding to the LexA-ERα(LBD), this acts as a transcription factor and binds to LexA operator sequences (Lex6Op) present in our reporter module expressing NanoLuc.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1606
Illegal PstI site found at 1790
Illegal PstI site found at 1961 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1790
Illegal PstI site found at 1961 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1740
Illegal BamHI site found at 73
Illegal BamHI site found at 390 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1606
Illegal PstI site found at 1790
Illegal PstI site found at 1961 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1606
Illegal PstI site found at 1790
Illegal PstI site found at 1961 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |