Part:BBa_K5477027
pRET2-LexA-ERα(LBD) - receptor module
The pRET2-LexA-ERα(LBD) composite part integrates the LexA DNA-binding domain (DBD) with the ligand-binding domain (LBD) of Estrogen Receptor Alpha (ERα), under the control of the pRET2 promoter. This fusion protein allows for the precise regulation of gene expression in response to estrogen or estrogen-like molecules, such as bisphenol A (BPA). The pRET2 promoter controls the expression of this fusion protein, ensuring that the LexA-ERα(LBD) is expressed under specific conditions determined by the regulatory elements of the pRET2 sequence. Upon presence of BPA, the LexA-ERα(LBD) binds specifically to LexA operator sequences (Lex6Op) which is a part of our reporter module which initiates transcription of NanoLuc.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1606
Illegal PstI site found at 1790
Illegal PstI site found at 1961 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 182
Illegal PstI site found at 1790
Illegal PstI site found at 1961 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1740
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1606
Illegal PstI site found at 1790
Illegal PstI site found at 1961 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1606
Illegal PstI site found at 1790
Illegal PstI site found at 1961
Illegal AgeI site found at 37 - 1000COMPATIBLE WITH RFC[1000]
None |