Composite

Part:BBa_K5477027

Designed by: Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-26)
Revision as of 19:56, 26 September 2024 by Registry (Talk | contribs)

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pRET2-LexA-ERα(LBD) - receptor module

The pRET2-LexA-ERα(LBD) composite part integrates the LexA DNA-binding domain (DBD) with the ligand-binding domain (LBD) of Estrogen Receptor Alpha (ERα), under the control of the pRET2 promoter. This fusion protein allows for the precise regulation of gene expression in response to estrogen or estrogen-like molecules, such as bisphenol A (BPA). The pRET2 promoter controls the expression of this fusion protein, ensuring that the LexA-ERα(LBD) is expressed under specific conditions determined by the regulatory elements of the pRET2 sequence. Upon presence of BPA, the LexA-ERα(LBD) binds specifically to LexA operator sequences (Lex6Op) which is a part of our reporter module which initiates transcription of NanoLuc.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1606
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 182
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1740
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1606
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1606
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
    Illegal AgeI site found at 37
  • 1000
    COMPATIBLE WITH RFC[1000]


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