Coding

Part:BBa_K5477015

Designed by: Kate Malana Escobar, Kristine Aspehaug Høyen, Thanh Le   Group: iGEM24_UCopenhagen   (2024-09-26)
Revision as of 16:41, 26 September 2024 by Registry (Talk | contribs)

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LexA-ERRγ Chimeric activator with LexA DNA binding domain fused with LBD of ERRγ

The LexA-ERRγ (LexA-Estrogen-Related Receptor Gamma) fusion protein combines the DNA-binding domain (DBD) of the LexA repressor with the ligand-binding domain (LBD) of Estrogen-Related Receptor Gamma (ERRγ), a member of the orphan nuclear receptor family. ERRγ is classified as an orphan receptor because, unlike classic nuclear receptors, it does not have a well-characterized endogenous ligand. Instead, it functions in a ligand-independent manner or is activated by synthetic or environmental ligands, allowing it to regulate a broad range of physiological processes, including energy metabolism, mitochondrial function, and cellular differentiation.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 856
    Illegal PstI site found at 1003
    Illegal PstI site found at 1150
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 856
    Illegal PstI site found at 1003
    Illegal PstI site found at 1150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 698
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 856
    Illegal PstI site found at 1003
    Illegal PstI site found at 1150
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 856
    Illegal PstI site found at 1003
    Illegal PstI site found at 1150
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None