Composite

Part:BBa_K5115033

Designed by: Yi Shi   Group: iGEM24_Fudan   (2024-09-20)
Revision as of 15:50, 26 September 2024 by Chenliyue (Talk | contribs)


ribozyme+RBS+RcnR_C35L+stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of RcnR_C35L and improved ribozyme-assisted polycistronic co-expression system: pRAP. The RcnR_C35L is a protein that can influence the transport of nickel ions, get details about RcnR_C35L in BBa_K5115000(RcnR_C35L).

Usage and Biology

The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 303
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

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Categories
Parameters
None