Introduction

Synthetic biology constitutes an effort towards making biology easy to engineer [1]. This means that basic principles of engineering find their place in biological systems; We treat biomers as spare, interchangeable parts, the same way that we would approach the construction of any mechanical device. This necessitates the standardization of parts, in order to establish objectivity regarding their effectiveness and to promote the acceleration of knowledge [2,3]. For our project, to identify the optimal components and regulatory mechanisms for our system, we employed the Design-Build-Test-Learn cycle, allowing us to manipulate the expression of our constructs throughout different phases of the bacterial life cycle. To minimize cellular stress, we strategically divided our system into two phases: the exponential phase and the stationary phase. During the exponential phase, we expressed T7 polymerase and dsRNA molecules. In our pursuit of an autoinducible promoter active during the exponential phase, we explored the work of Zobel et al. and tested three of their synthetic promoters. Through our focused examination of their autoinducible properties, we determined that BG37 emerged as the optimal choice for regulating our system and we decided to further characterize BG37 by assessing its performance across various bacterial chassis, employing different plasmid backbones and carbon sources. By thoroughly characterizing this new basic part, we believe it will serve as a valuable tool for future teams aiming for orthogonal expression during the exponential phase.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Sequence and Features

Part:BBa_K5299008

Introduction