Coding

Part:BBa_K5332003:Design

Designed by: Qilin Yu   Group: iGEM24_NKU-China   (2024-09-26)
Revision as of 15:25, 26 September 2024 by Registry (Talk | contribs)

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CMC (arttificial adhesion protein)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 470
    Illegal PstI site found at 673
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1444
    Illegal PstI site found at 470
    Illegal PstI site found at 673
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 470
    Illegal PstI site found at 673
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 470
    Illegal PstI site found at 673
    Illegal NgoMIV site found at 381
    Illegal NgoMIV site found at 1806
    Illegal NgoMIV site found at 1810
    Illegal AgeI site found at 295
    Illegal AgeI site found at 1396
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

OmpA, which helps the protein to cross the cell membrane, needs to be attached to the N-terminus of the protein to enable CMC to localize on the membrane surface to play an adhesive role after expression in the cell. The copy number of CBMcipc may affect its ability to bind dextran, so different copy numbers of the factor should be designed for adhesion testing, and also the copy number of CBMcipc may have an effect on its cellular distribution, and experiments need to be designed to detect the cellular distribution of the factor.



Source

The core component of CMC, CBMcipc, is derived from the scaffold protein CipC of Clostridium cellulolyticum

References