Coding

Part:BBa_K5276001:Design

Designed by: Zitao Qiu   Group: iGEM24_UCAS-China   (2024-09-26)
Revision as of 14:40, 26 September 2024 by Qiuzitao (Talk | contribs) (→‎References)


A118 recombinase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 325
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 325
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 325
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 325
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 721


Design Notes

Source

References

Mandali, S., Gupta, K., Dawson, A. R., Van Duyne, G. D., & Johnson, R. C. (2017). Control of Recombination Directionality by the Listeria Phage A118 Protein Gp44 and the Coiled-Coil Motif of Its Serine Integrase. J Bacteriol, 199(11). doi:10.1128/JB.00019-17 Roquet, N., Soleimany, A. P., Ferris, A. C., Aaronson, S., & Lu, T. K. (2016). Synthetic recombinase-based state machines in living cells. Science, 353(6297), aad8559. doi:10.1126/science.aad8559