Coding
RFC 37

Part:BBa_K245116:Design

Designed by: Nika Debeljak   Group: iGEM09_Slovenia   (2009-10-14)
Revision as of 18:22, 21 October 2009 by Spela (Talk | contribs) (→‎Source)

GyrB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 428
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
    Illegal AgeI site found at 238
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37.


Source

The part was multiplied by PCR from GyrB-L20-MD2/pFLAG-CMV3.

References