Part:BBa_K5382160
InaK-linker-4z domain_protein domain that have the ability to bind to lgG
Inak is a kind of ice nucleoprotein of Pseudomonas syringae KCTC1832. Ice nucleoprotein is a kind of protein that can promote the formation of ice crystals in organisms. Its N-terminal domain can be tightly anchored to the surface of E. coli cells as an anchor moda, so as to enable surface display. linker here is a short peptide sequence used to connect the Inak and z domains. z domain refers to a synthetic protein domain that has the ability to bind to the Fc region of immunoglobulin G (IgG). In studies, z domains are used to construct extracellular vesicles (EVs) that can display antibodies, so that these EVs can bind specific antibodies through the z domain on their surface, so as to achieve targeted delivery to specific cells or tissues [1]. The 4z domain, a tetramer of the z domain, has been shown in the literature to be equally capable of constructing extracellular vesicles (EVs) displaying antibodies, and has similar binding activity to the z domain
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2299
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 722
Illegal NgoMIV site found at 962
Illegal NgoMIV site found at 1130
Illegal AgeI site found at 517 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 692
Illegal BsaI.rc site found at 1182
Illegal SapI.rc site found at 326
Illegal SapI.rc site found at 1951
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