Reporter
PFec-RFP
Part:BBa_K257023:Experience
Designed by: Romain Bodinier Group: iGEM09_Paris (2009-10-19)
Revision as of 18:08, 21 October 2009 by David.bikard (Talk | contribs)
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Caracterisation of BBa_K257023
Overnight cultures of BBa_K257023 were diluted 1/100 in LB or M9 medium supplemented with varing amont of FeNO3 and Na Citrate. Fluorescence and OD were assessed in a Tecan plate reader. Fluorescence divided by OD is ploted.
The low Fluo/OD at high FeNO3 concentration can be explained by the taxicity of FeNO3. Note that the best response to Na Citrate is obtained with 1mM FeNO3.
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