Coding

Part:BBa_K5184031

Designed by: SHA ZHOU   Group: iGEM24_GreatBay-SCIE   (2024-09-26)
Revision as of 08:17, 26 September 2024 by Registry (Talk | contribs)

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G1M5-rCtx-his

In order to eliminate T.urticae of infested cultivations, spider venom peptide rCtx-4 is incorporated in our project with pesticidal means. rCtx-4 is a neurotoxin obtained from the ctenid spider Phoneutria depilata, naturally employed to incapacitate their prey. Having the voltage-gated sodium channels playing a vital role in neuronal, muscular and cardiac functions, targets experience fast immobilization after envenomation, thus it serves as an effective pesticide. Due to the cysteine-rich nature of rCtx-4, expression strategy that exterminates inclusion bodies is required, thus a G1M5 secretion system is engineered with the SVP to achieve this. Considering future pesticidal control, G1M5-rCtx-4-his can provide future iGEM teams that dedicate in exterminating other destructive pests more choices of sustainable pesticide that could be expressed correctly in Escherichia Coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None