Part:BBa_K5152004

PbrR-pPbr lead sensing chromoprotein reporter device

Our biosensor construct for detecting heavy metal lead is inspired by iGEM 2007 Team Brown (I721001). The regulatory protein PbrR controls the pPbr promoter. When lead is present, PbrR forms a dimer, activating the expression of downstream coding sequences.

We employed this construct in E. coli, which doesn't naturally express PbrR, so we co-expressed it. We used dTomato as the reporter gene, an RFP with chromoprotein properties, chosen for its visible color to avoid the need for expensive equipment. This sequence, optimized by our 2023 team, ensures vibrant and stable expression.

Our project examined the expression profiles of several chromoproteins, including amilCP, cjBlue, tsPurple, eforRed, and dTomato. For more details, please refer to our wiki page.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 35
  Illegal NheI site found at 58
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 714