Composite
Part:BBa_K5246043:Design
Designed by: Edgaras Zaboras Group: iGEM24_Vilnius-Lithuania (2024-09-25)
Caulobacter crescentus CB2/CB2A HfsE-HfsJ-HfsG-HfsH-HfsK-HfsL Polysaccharide tetrad assembly
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3690
Illegal XbaI site found at 4450
Illegal PstI site found at 4539 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3690
Illegal PstI site found at 4539
Illegal NotI site found at 619
Illegal NotI site found at 3444 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3690
Illegal BglII site found at 2280
Illegal BglII site found at 4453
Illegal BamHI site found at 2428
Illegal XhoI site found at 2647
Illegal XhoI site found at 5268 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3690
Illegal XbaI site found at 4450
Illegal PstI site found at 4539 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3690
Illegal XbaI site found at 4450
Illegal PstI site found at 4539
Illegal NgoMIV site found at 758
Illegal NgoMIV site found at 1389
Illegal NgoMIV site found at 2001
Illegal NgoMIV site found at 3099
Illegal NgoMIV site found at 3748
Illegal NgoMIV site found at 3772
Illegal NgoMIV site found at 3776
Illegal AgeI site found at 1088 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
NA
Source
NA