Coding

Part:BBa_K5382124

Designed by: yuyanyan chen   Group: iGEM24_HUBU-4-CHN   (2024-09-25)
Revision as of 15:14, 25 September 2024 by Registry (Talk | contribs)

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CL7-A protein tag

CL7 is an engineered variant of colicin CE7, which is a protein tag. In Escherichia coli, CE7 is a bacteriocin that belongs to the colicin DNase family and has endonuclease activity. Through engineering, CL7 removed DNA binding and catalytic activity, but retained its high affinity binding ability to the corresponding inhibitory protein Im7. This property makes CL7 a useful tool for protein purification and molecular biology applications.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 133
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 133
    Illegal BglII site found at 81
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 133
    Illegal AgeI site found at 31
    Illegal AgeI site found at 45
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None