Plasmid

Part:BBa_K5490035

Designed by: IOANNIS VASILEIOS ELAFROPOULOS   Group: iGEM24_IOANNINA   (2024-09-25)
Revision as of 15:14, 25 September 2024 by Registry (Talk | contribs)

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pCMV-Myc:mNFAT:TALA:VP1

Vector containing a synthetic NFAT with only a Myc tag

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 582
    Illegal EcoRI site found at 1742
    Illegal XbaI site found at 4713
    Illegal SpeI site found at 557
    Illegal SpeI site found at 9425
    Illegal PstI site found at 1560
    Illegal PstI site found at 1814
    Illegal PstI site found at 6058
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 582
    Illegal EcoRI site found at 1742
    Illegal NheI site found at 1131
    Illegal SpeI site found at 557
    Illegal SpeI site found at 9425
    Illegal PstI site found at 1560
    Illegal PstI site found at 1814
    Illegal PstI site found at 6058
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 582
    Illegal EcoRI site found at 1742
    Illegal BglII site found at 9188
    Illegal BamHI site found at 551
    Illegal BamHI site found at 1609
    Illegal BamHI site found at 4359
    Illegal XhoI site found at 1848
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 582
    Illegal EcoRI site found at 1742
    Illegal XbaI site found at 4713
    Illegal SpeI site found at 557
    Illegal SpeI site found at 9425
    Illegal PstI site found at 1560
    Illegal PstI site found at 1814
    Illegal PstI site found at 6058
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 582
    Illegal EcoRI site found at 1742
    Illegal XbaI site found at 4713
    Illegal SpeI site found at 557
    Illegal SpeI site found at 9425
    Illegal PstI site found at 1560
    Illegal PstI site found at 1814
    Illegal PstI site found at 6058
    Illegal NgoMIV site found at 1014
    Illegal NgoMIV site found at 1034
    Illegal NgoMIV site found at 1050
    Illegal NgoMIV site found at 1515
    Illegal NgoMIV site found at 1799
    Illegal NgoMIV site found at 5167
    Illegal NgoMIV site found at 6509
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 526
    Illegal BsaI.rc site found at 8320
    Illegal SapI site found at 7237
    Illegal SapI.rc site found at 4703
    Illegal SapI.rc site found at 6358
    Illegal SapI.rc site found at 6568


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