Part:BBa_K5143002

Cp19k : a barnacle cement protein used as a bioglue, optimised for Saccharomyces cerevisiae

Description

Barnacle, a marine organism, easily stick to underwater matrices through the secretion of various proteins and forming cement complexes. The protein complex for barnacle, Cp19k plays a key role in interfacial adhesion. Adhesion strength: 2.2 mJ/m². See BBa_K3089008 which provided the inspiration for the discovery of this sticky protein.

Figure 1: Cp19k Gene

Figure 2: Barnacle on a rock surface

Construction

The Cp19k gene was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with spider silk protein as a bioglue, to improve its adhesive properties: BBa_K5143003

References

Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]