Part:BBa_K5241008

pPICZaAPkrhdd-6xH

PICZα" refers to a vector containing the yeast alpha-factor signal peptide sequence, which aids in the proper secretion of proteins. It was developed by Invitrogen (now part of Thermo Fisher Scientific). "pPICZαA-PHDD-6xH" is a vector used for expressing the PHDD protein with a 6xHis tag in Pichia pastoris

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 43
  Illegal EcoRI site found at 52
  Illegal EcoRI site found at 967
  Illegal XbaI site found at 5174
  Illegal PstI site found at 925
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 43
  Illegal EcoRI site found at 52
  Illegal EcoRI site found at 967
  Illegal NheI site found at 4469
  Illegal PstI site found at 925
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 43
  Illegal EcoRI site found at 52
  Illegal EcoRI site found at 967
  Illegal BglII site found at 1089
  Illegal BglII site found at 2620
  Illegal BamHI site found at 2977
  Illegal XhoI site found at 1075
  Illegal XhoI site found at 2284
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 43
  Illegal EcoRI site found at 52
  Illegal EcoRI site found at 967
  Illegal XbaI site found at 5174
  Illegal PstI site found at 925
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 43
  Illegal EcoRI site found at 52
  Illegal EcoRI site found at 967
  Illegal XbaI site found at 5174
  Illegal PstI site found at 925
  Illegal NgoMIV site found at 342
  Illegal AgeI site found at 319
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 5330
  Illegal BsaI.rc site found at 316
  Illegal BsaI.rc site found at 3129