Coding

Part:BBa_K211002

Designed by: ZHONG Wanting   Group: iGEM09_HKUST   (2009-10-13)
Revision as of 17:34, 21 October 2009 by Siduri (Talk | contribs)

RI7-odr10 chimeric GPCR

BBa_K211002 encodes a chimeric diacetyl-sensing GPCR which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the TM2-TM7 ligand-binding domain of the C. elegans 7-transmembrane receptor ODR-10, flanked by the N- and C- terminals of the rat OR RI7. It can be localized to the S. cerevisiae cell membrane and activate the yeast endogenous MAPK pathway upon binding to the ligand diacetyl.

File:Receptor.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 761
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/receptor
//chassis/eukaryote/yeast
//function/cellsignalling
Parameters
chassisyeast
familyGPCR
functiondiacetyl sensing GPCR
ligandsdiacetyl
originC.elegans ODR-10 and Rat RI7
tagFLAG