DNA

Part:BBa_K5061215:Design

Designed by: Florent Cha   Group: iGEM24_Evry-Paris-Saclay   (2024-09-25)
Revision as of 12:10, 25 September 2024 by Chaflorent (Talk | contribs)

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∆ung repair DNA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 731
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 731
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 731
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 731
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

see main page of this part


Source

PCR aplification from E. coli MG1655* ∆flu ∆ung ∆nfi ∆pyrF described by Álvarez et al., 2020.


References

Álvarez B, Mencía M, de Lorenzo V, Fernández LÁ. In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9. Nature Communications (2020) 11: 6436.