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10XTALE

Part:BBa_K5490027

Designed by: IOANNIS VASILEIOS ELAFROPOULOS   Group: iGEM24_IOANNINA   (2024-09-25)
Revision as of 10:36, 25 September 2024 by Registry (Talk | contribs)

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TALE BINDING SITE FOR SYNTHETIC NFAT

TALEs can be designed to bind almost any sequence of choice. Furthermore, one TALE sequence can target multiple TALE binding sites, provided the proximity between the sites is sufficiently low. These binding sites can be positioned anywhere, whether in central or peripheral DNA regions.

In this particular case, a TALE sequence is fused with the NFAT transcription factor to bind to 10 TALE binding site upstream of a minimal promoter. Upon receiving a stimulus, this setup activates transcription with high specificity


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 141
    Illegal NotI site found at 211
    Illegal NotI site found at 506
    Illegal NotI site found at 576
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 7
    Illegal AgeI site found at 347
    Illegal AgeI site found at 372
    Illegal AgeI site found at 712
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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