Composite
Part:BBa_K5061116:Design
Designed by: Florent Cha Group: iGEM24_Evry-Paris-Saclay (2024-09-25)
nfi gRNA transcription unit under the control of the J23119(SpeI) promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 30
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal SpeI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 30
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 30
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
to do
Source
DNA synthesis and assembly