Composite

Part:BBa_K5301029

Designed by: Zihan Wen   Group: iGEM24_BNU-China   (2024-09-25)
Revision as of 08:39, 25 September 2024 by Registry (Talk | contribs)

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spNW15 expression pathway (T7)

This pathway expresses the SpyCatcher-NW15-SpyTag fusion protein under IPTG induction, achieving self-cyclization of NW15 to construct nanodiscs.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 571
    Illegal SpeI site found at 523
    Illegal PstI site found at 739
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 571
    Illegal NheI site found at 139
    Illegal NheI site found at 1175
    Illegal SpeI site found at 523
    Illegal PstI site found at 739
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 571
    Illegal BglII site found at 627
    Illegal BglII site found at 999
    Illegal BamHI site found at 172
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 571
    Illegal SpeI site found at 523
    Illegal PstI site found at 739
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 571
    Illegal SpeI site found at 523
    Illegal PstI site found at 739
    Illegal AgeI site found at 1102
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None