Plasmid

Part:BBa_K5392015

Designed by: ZHENQIANG SUN   Group: iGEM24_LIUAN-Nanjing   (2024-09-24)
Revision as of 08:27, 25 September 2024 by Zoezhuang (Talk | contribs)


Vector-ZaTdT-KanR

Here, we loaded the genes encoding TdT into plasmid vectors. In this way we were able to over-express the ZaTdT in the Escherichia coli BL21.

Description

We transfected the ZaTdT gene-bonded pET28a plasmid into E.coli BL21(DE3) competent cell. After overnight, Colonies were then picked and performs protein expression. We identified the ZaTdT expression by SDS-PAGE and tested the incorporation of modified nucleotides by purified ZaTdT with polyacrylamide gel electrophoresis.

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