Part:BBa_K191005

TRP promoter - RBS - TetR - Term - TetR promoter - RBS - RFP - Term

Part BBa_K191005 is a double-repressor system: there is a Trp promoter in front of the TetR gene, followed by the Tet promoter in front of the RFP gene. This means that it should react both to tryptophan and tetracycline, an allosteric blocker of TetR (for our experiments we used anhydrous tetracycline, ATC).

Usage and Biology

This part permits characterization of part BBa_K191003 as it contains Trp promoter sequence, to which the fused protein LovTAP can bind. For complete genetic circuit, see here.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 43
  Illegal SpeI site found at 51
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 43
  Illegal SpeI site found at 51
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 43
  Illegal SpeI site found at 51
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 43
  Illegal SpeI site found at 51
  Illegal AgeI site found at 1576
  Illegal AgeI site found at 1688
  
• 1000
  COMPATIBLE WITH RFC[1000]