Composite

Part:BBa_K5186007

Designed by: Jiawen Chen   Group: iGEM24_AIS-China   (2024-09-25)
Revision as of 03:28, 25 September 2024 by Registry (Talk | contribs)

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PTac-riboJ-DXS-IspG-DXR-B0015

PTac-riboJ-DXS-IspG-DXR-B0015 is an expression cassette in E. coli expressing DXS (BBa_K3166061), IspG (BBa_K1653001) and DXR(BBa_K51860010) used to overexpress methylerythritol phosphate(MEP) pathway. With this expression cassette, DXS, IspG and DXR can be IPTG-inducibly expressed under the control of pTac-RiboJ (BBa_K3552015) in all strains of E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3332
    Illegal XbaI site found at 132
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3332
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3332
    Illegal BglII site found at 2474
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3332
    Illegal XbaI site found at 132
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3332
    Illegal XbaI site found at 132
    Illegal AgeI site found at 1392
    Illegal AgeI site found at 3631
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 886
    Illegal SapI.rc site found at 2388


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