Translational_Unit
LucTS

Part:BBa_K5490023

Designed by: IOANNIS VASILEIOS ELAFROPOULOS   Group: iGEM24_IOANNINA   (2024-09-25)
Revision as of 00:35, 25 September 2024 by Registry (Talk | contribs)

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LUCIFERASE WITH TARGET SITES IN

A luciferase reporter containing three target sequences, each 23 nucleotides long and representing specific loci in the WNV genome, has been designed using an algorithmic approach to identify optimal CasRx activity targets. Researchers can modify these sequences to experimentally assess the cleavage efficiency of their own target sequences through a luciferase assay

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1693
    Illegal XbaI site found at 1915
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1693
    Illegal NotI site found at 1898
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1693
    Illegal BamHI site found at 1906
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1693
    Illegal XbaI site found at 1915
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1693
    Illegal XbaI site found at 1915
    Illegal NgoMIV site found at 74
    Illegal NgoMIV site found at 1451
    Illegal NgoMIV site found at 1472
    Illegal NgoMIV site found at 1787
    Illegal AgeI site found at 1166
  • 1000
    COMPATIBLE WITH RFC[1000]


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