Part:BBa_K195623

CII/PenI Damped Oscillator With GFP Reporter (II)

This device is produces damped oscillations between PenI and CII. The pRE (BBa_K116603) can be activated by CII (BBa_K116602), which in turn produces PenI (BBa_C0074) and GFP. The PenI then represses the pPenI promoter (BBa_R0074) resulting in slower production of CII. This design was based on the design of a relaxation oscillator (two component oscillator). This design does not produce sustained oscillations like a normal oscillator (relaxation based or otherwise) because of the two oscillating effectors not being able to directly affect each other.

Modelling

The modeling graph would look like the same as the CII/TetR damped oscillator with the only difference being that the rate of damping would occur faster if the (strength of pPenI)/(strength of CII) is smaller.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 516
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 334
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1927