Translational_Unit

Part:BBa_K5490017

Designed by: IOANNIS VASILEIOS ELAFROPOULOS   Group: iGEM24_IOANNINA   (2024-09-24)
Revision as of 20:25, 24 September 2024 by Registry (Talk | contribs)

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Ca-Dependent Synthetic NF-AT

Is a synthetic NFAT transcription factor, after an increase in calcium, will enter the nucleus and bind to a specific minimal promoter. It uses the TALE system for binding to DNA and VP16 as the activation domain. Under homeostatic conditions, it is anchored in the plasma membrane via the KRφ peptide. It has both Myc and FLAG tags for immunohistochemical analysis and Western blotting

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 723
    Illegal EcoRI site found at 766
    Illegal EcoRI site found at 1926
    Illegal SpeI site found at 741
    Illegal PstI site found at 1744
    Illegal PstI site found at 1998
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 723
    Illegal EcoRI site found at 766
    Illegal EcoRI site found at 1926
    Illegal NheI site found at 1315
    Illegal SpeI site found at 741
    Illegal PstI site found at 1744
    Illegal PstI site found at 1998
    Illegal NotI site found at 715
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 723
    Illegal EcoRI site found at 766
    Illegal EcoRI site found at 1926
    Illegal BamHI site found at 1793
    Illegal BamHI site found at 4543
    Illegal XhoI site found at 2032
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 723
    Illegal EcoRI site found at 766
    Illegal EcoRI site found at 1926
    Illegal SpeI site found at 741
    Illegal PstI site found at 1744
    Illegal PstI site found at 1998
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 723
    Illegal EcoRI site found at 766
    Illegal EcoRI site found at 1926
    Illegal SpeI site found at 741
    Illegal PstI site found at 1744
    Illegal PstI site found at 1998
    Illegal NgoMIV site found at 1198
    Illegal NgoMIV site found at 1218
    Illegal NgoMIV site found at 1234
    Illegal NgoMIV site found at 1699
    Illegal NgoMIV site found at 1983
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None