Composite

Part:BBa_K283022:Design

Designed by: Nelson Chu, W Ling , Shi Lei   Group: iGEM09_HKU-HKBU   (2009-10-13)
Revision as of 17:05, 21 October 2009 by NelsonChu (Talk | contribs) (→‎Source)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

pLacI-rbs-cheZ

The BBa_K283016 has been made from this composite. The backbone of this biobrick is based on BBa_J61003. First, we changed the pTet-rbs into pLacI-rbs. Then the cheZ encoding gene was inserted between XbaI and SpeI.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 872
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

This part is composited from BBa_R0010, BBa_B0034 and BBa_K094100

References