RNA

Part:BBa_K228001

Designed by: Lin Min   Group: iGEM09_PKU_Beijing   (2009-08-25)
Revision as of 17:02, 21 October 2009 by Supergrc (Talk | contribs)

SupD-tRNA

The SupD-tRNA part functions as a tRNA, it suppresses the TAG amber stop codon and translate it into a Ser. There is no need to assembly SupD-tRNA with a rbs, since it is not translated.

This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 33
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 36

Reference Anderson JC, Voigt CA, and Arkin AP. Environmental signal integration by a modular AND gate. Molecular Systems Biology. 2007, 3:133


[edit]
Categories
Parameters
n/aSupD-tRNA