RNA
SPACER2WNV

Part:BBa_K5490001:Design

Designed by: IOANNIS VASILEIOS ELAFROPOULOS   Group: iGEM24_IOANNINA   (2024-09-23)
Revision as of 16:26, 24 September 2024 by Tzonissss13 (Talk | contribs) (→‎Design Notes)


23-nt sequence binds CasRx to cleave WNV genome; modifiable target 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Spacer Sequences: These sequences are complementary to specific regions of the virus and guide the endonuclease activity of CasRx to its target.

Source

Soure:Silvana Konermann Laboratory of Molecular and Cell Biology, Salk Institute for Biological Studies, 10010 N Torrey Pines Rd, La Jolla, CA 92037, USA Direct repeat sequence,firstly identified at Ruminococcus Spacer sequence desingned by iGEM IOANNINA 2024 dry lab team

References