Protein_Domain
mNFAT

Part:BBa_K5490006

Designed by: IOANNIS VASILEIOS ELAFROPOULOS   Group: iGEM24_IOANNINA   (2024-09-24)
Revision as of 12:31, 24 September 2024 by Registry (Talk | contribs)

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mNFAT

murine NFAT regulatory domain In its natural state, NFAT contains two Nuclear Localization Signals (NLS) that enable nuclear translocation. However, in this engineered version, one NLS has been removed, leaving only a single NLS to reduce background transcription. The NFAT domain is activated through phosphorylation by calcineurin in response to calcium signaling. This domain is critical for calcium-dependent activation in cellular processes, as the increase in cytosolic calcium activates calmodulin, which, in turn, activates calcineurin.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1116
    Illegal PstI site found at 934
    Illegal PstI site found at 1188
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1116
    Illegal NheI site found at 505
    Illegal PstI site found at 934
    Illegal PstI site found at 1188
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1116
    Illegal BamHI site found at 983
    Illegal XhoI site found at 1222
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1116
    Illegal PstI site found at 934
    Illegal PstI site found at 1188
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1116
    Illegal PstI site found at 934
    Illegal PstI site found at 1188
    Illegal NgoMIV site found at 388
    Illegal NgoMIV site found at 408
    Illegal NgoMIV site found at 424
    Illegal NgoMIV site found at 889
    Illegal NgoMIV site found at 1173
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain
Parameters
None