Coding

Part:BBa_K5374023:Design

Designed by: Langning Xu   Group: iGEM24_TestDaily-I-Beijing   (2024-09-24)
Revision as of 11:57, 24 September 2024 by Francisgrace (Talk | contribs)

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VWF-A Domain-EGFP (von Willebrand Factor A Domain). A domain known for mediating collagen binding, p


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 112
    Illegal PstI site found at 875
    Illegal PstI site found at 1282
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1330
    Illegal PstI site found at 112
    Illegal PstI site found at 875
    Illegal PstI site found at 1282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 849
    Illegal BamHI site found at 1017
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 112
    Illegal PstI site found at 875
    Illegal PstI site found at 1282
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 112
    Illegal PstI site found at 875
    Illegal PstI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1973


Design Notes

The sequence was optimized for EGFP fusion, ensuring the domain’s collagen-binding function while maintaining fluorescence for activity evaluation.


Source

VWF-A domain from von Willebrand factor, with EGFP from BBa_K1911005.