Composite

Part:BBa_K5186021

Designed by: Jiawen Chen   Group: iGEM24_AIS-China   (2024-09-24)
Revision as of 11:29, 24 September 2024 by Registry (Talk | contribs)

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Ptac-RiboJ-RBS-X174E-B0015

Ptac-RiboJ-RBS-X174E-B0015 is an expression cassette in E. coli expressing gene E(BBa_K1835500) from the ΦX174 bacteriophage, which encodes a transmembrane pore-forming protein that can cleave the host cell membrane to induce the leakage of intracellular proteins. With this expression cassette, gene E can be IPTG-inducibly expressed under the control of pTac-RiboJ (BBa_K3552015) in all strains of E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None