Composite
Part:BBa_K5186021
Designed by: Jiawen Chen Group: iGEM24_AIS-China (2024-09-24)
Ptac-RiboJ-RBS-X174E-B0015
Ptac-RiboJ-RBS-X174E-B0015 is an expression cassette in E. coli expressing gene E(BBa_K1835500) from the ΦX174 bacteriophage, which encodes a transmembrane pore-forming protein that can cleave the host cell membrane to induce the leakage of intracellular proteins. With this expression cassette, gene E can be IPTG-inducibly expressed under the control of pTac-RiboJ (BBa_K3552015) in all strains of E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |