Coding

Part:BBa_K5186020

Designed by: Jiawen Chen   Group: iGEM24_AIS-China   (2024-09-24)
Revision as of 10:58, 24 September 2024 by Registry (Talk | contribs)

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2Pa-T4L

he 2Pa-T4L, encoding for a fusion protein of two amphipathic Pa peptides and T4 phage lysozyme(T4L), a lytic protein possesses the ability to degrade peptidoglycan, effectively targeting E. coli, thereby causing the autolysis of E. coli. Each Pa peptide is fused to the N-terminus of T4L via a flexible GSA peptide linker(Sequence: GSAGSAAGSG). It is reported that T4L's lytic process can be enhanced by fusing cell-penetrating peptides. And the efficiency of lysis is significantly influenced by the sequence and quantity of the incorporated cell-penetrating peptides.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
    Illegal AgeI site found at 426
    Illegal AgeI site found at 496
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None