Coding

Part:BBa_K5301022

Designed by: Jiaxin Cao   Group: iGEM24_BNU-China   (2024-09-24)
Revision as of 10:24, 24 September 2024 by Registry (Talk | contribs)

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SpyCatcher-spNW50-SpyTag achieving self-cyclization of MSP nanodisc.

This pathway expresses the SpyCatcher-NW50-SpyTag fusion protein under IPTG induction, achieving self-cyclization of NW50 to construct nanodiscs.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1645
    Illegal SpeI site found at 517
    Illegal PstI site found at 733
    Illegal PstI site found at 1273
    Illegal PstI site found at 1306
    Illegal PstI site found at 1813
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1645
    Illegal NheI site found at 133
    Illegal NheI site found at 2061
    Illegal SpeI site found at 517
    Illegal PstI site found at 733
    Illegal PstI site found at 1273
    Illegal PstI site found at 1306
    Illegal PstI site found at 1813
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1645
    Illegal BglII site found at 993
    Illegal BglII site found at 1533
    Illegal BglII site found at 1701
    Illegal BamHI site found at 166
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1645
    Illegal SpeI site found at 517
    Illegal PstI site found at 733
    Illegal PstI site found at 1273
    Illegal PstI site found at 1306
    Illegal PstI site found at 1813
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1645
    Illegal SpeI site found at 517
    Illegal PstI site found at 733
    Illegal PstI site found at 1273
    Illegal PstI site found at 1306
    Illegal PstI site found at 1813
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None